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| Gouveia,E.M.M.F.; Silva,I.S.; Nakazato,G.; Onselem,V.J.V.; Corrêa,R.A.C.; Araujo,F.R.; Chang,M.R.. |
| The therapeutic action of phosphorylated mannanoligosaccharides (MOS) was investigated regarding its prebiotic activity on enteropathogenic Escherichia coli (EPEC). Diarrhea was induced in dogs by experimental infection with EPEC strains. Then MOS was supplied once a day, in water for 20 days. Immunological (IgA and IgG), hematological (lymphocytes, neutrophils and monocytes) and bacteriological variables (PCR detection of the eae gene of EPEC recovered from stool culture), as well as occurrence of diarrhea were evaluated. All strains caused diarrhea at 24, 48 and 72 h after infection. PCR results indicated that E. coli isolated from stool culture of all infected animals had the eae gene. There was no significant difference among groups as to number of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteropathogenic E. coli; In vivo experiment; Dogs; Phosphorylated 31 mannanoligo saccharides; PCR. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200027 |
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| Rossato,J.M.; Brito,B.G.; Kobayashi,R.K.T.; Koga,V.L.; Sarmiento,J.J.P.; Nakazato,G.; Lopes,L.F.D.; Balsan,L.A.G.; Grassotti,T.T.; Brito,K.C.T.. |
| ABSTRACT Diarrheagenic (DEC) and avian pathogenic Escherichia coli (APEC) are associated with intestinal and extra-intestinal infections (ExPEC), respectively. We aimed to analyze the antimicrobial susceptibility, gene encoding virulence factors associated to DEC and APEC, and phylogenetic classification in E. coli isolated from 320 samples of feed and ingredients. Antimicrobial susceptibility was performed using the disk diffusion method and Multiple Antibiotic Resistance (MAR) Index and Multi-Drug Resistance (MDR) were calculated. Phylogenetic classification was performed on samples harboring DEC and/or APEC virulence-associated genes. A total of 110 E. coli strains were isolated in 15% (49/320) of the evaluated inputs (n=13 vegetable meal; n=33 animal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Poultry production; Animal feed; Antimicrobials; Virulence; Resistance. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601968 |
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| Penatti,M.P.A.; Hollanda,L.M.; Nakazato,G.; Campos,T.A.; Lancellotti,M.; Angellini,M.; Brocchi,M.; Rocha,M.M.M.; Silveira,W. Dias da. |
| Shigella spp are Gram-negative, anaerobic facultative, non-motile, and non-sporulated bacilli of the Enterobacteriaceae family responsible for "Shigellosis" or bacillary dysentery, an important cause of worldwide morbidity and mortality. However, despite this, there are very few epidemiological studies about this bacterium in Brazil. We studied the antibiotic resistance profiles and the clonal structure of 60 Shigella strains (30 S. flexneri and 30 S. sonnei) isolated from shigellosis cases in different cities within the metropolitan area of Campinas, State of São Paulo, Brazil. We used the following well-characterized molecular techniques: enterobacterial repetitive intergenic consensus, repetitive extragenic palindromic, and double-repetitive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella flexneri; Shigella sonnei; Antimicrobial resistance; Enterobacterial repetitive intergenic consensus- polymerase chain reaction; Repetitive extragenic palindromic-polymerase chain reaction; Double-repetitive element-polymerase chain reaction. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200012 |
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